发明名称 |
PHOTOACTIVATED CHEMICAL BLEACHING OF DYES |
摘要 |
Methods comprising the use of photoactivated chemical bleaching for detecting multiple targets in a biological sample are provided. The methods include the steps of providing a biological sample including multiple targets, binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The method further includes the steps of contacting the sample comprising the bound probe with an electron transfer reagent, as well as an optional additive which prevents target modification during photoactivated chemical bleaching, and irradiating the sample, thereby initiating a photoreaction that substantially inactivates the probe by photoactivated chemical bleaching. The method further includes the steps of binding at least one probe to one or more target present in the sample, and detecting a signal from the probe. The process of binding, detecting and bleaching may be iteratively repeated. |
申请公布号 |
WO2014093455(A1) |
申请公布日期 |
2014.06.19 |
申请号 |
WO2013US74328 |
申请日期 |
2013.12.11 |
申请人 |
CLARIENT DIAGNOSTIC SERVICES, INC. |
发明人 |
NATARAJAN, ARUNKUMAR;FILKINS, ROBERT JOHN;SOOD, ANUP;KAANUMALLE, LAKSHMI SIREESHA;SHAIKH, KASHAN ALI |
分类号 |
G01N33/542;G01N33/58 |
主分类号 |
G01N33/542 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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