Fabrication Of Bone Regeneration Scaffolds And Bone Filler Material Using A Perfusion Flow System

摘要

The present disclosure is directed at a process to form bone grafting material. One may provide a porous collagen scaffold and insert the scaffold into a perfusion chamber of a perfusion flow system. This may then be followed by continuously providing a mineralization perfusion fluid flow through the scaffold at a flow rate to provide dynamic intrafibrillar mineralization of the scaffold and form a collagen/hydroxyapatite composite scaffold. One may optionally provide the scaffold with bone tissue forming cells and then deliver a perfusion fluid including oxygen and one or more nutrients through the collagen/hydroxyapatite composite scaffold and to the bone tissue forming cells at a flow rate such that the bone tissue forming cells remodel the collagen/hydroxyapatite composite scaffold and form a bone tissue extracellular matrix. The bone tissue extracellular matrix may then be decellularized to form an acellular bone repair scaffold.

主权项

1. A process to form a bone grafting material, comprising:
providing a porous collagen scaffold; inserting the collagen scaffold into a perfusion chamber of a perfusion flow system; continuously providing a mineralization perfusion fluid flow through the collagen scaffold at a flow rate to provide dynamic intrafibrillar mineralization of the collagen scaffold and forming a collagen/hydroxyapatite composite scaffold.