| 摘要 |
The invention relates to the field of molecular and cell biology. Proposed is a method for influencing the proliferative status of cells using specific G-chain oligonucleotide sequences of human telomeric DNA, said sequences imitating open forms of telomeric overhangs in cells, where, unlike the traditional concept of the cytostatic use thereof, they are seen not as signals of DNA damage, but rather as molecular and quantum-optical signals regulating the transition between phases of the cell cycle. The invention allows for significantly increasing the specificity and effectiveness of telomere DNA-therapy by means of using specific nucleotide sequences on the basis of six variations of triplet endings (SEQ ID NO: 7-12). The ending SEQ ID NO: 8 comprises the basis for the effect which suppresses division, and the ending SEQ ID NO: 12 is at the foundation of stimulating cell proliferation. The effectiveness of the influence of the endings is regulated by selecting the length and initial nucleotides of the sequences, which determine the base variations of telomeric repetition (SEQ ID NO: 1-6). Forming corrective mixtures of triplet endings of the telomere oligonucleotides on the basis of an initial determination of the terminal nucleotides of the telomere DNA in cells allows for compensating for insufficient endings at specific stages of the cell cycle. For malignant cells, this provides a normal progression and duration of division. The invention is intended for influencing the proliferative status of cells, with the goal of suppressing or stimulating division, and also for controlling the time at which certain phases of the cell cycle come about, and can be used in medicine for treating oncological diseases, in immunology for controlling the body's immune status, and in transplantology, gerontology and cosmetology for maintaining the ability of cells to regenerate and rejuvenate the body. |
