发明名称 |
METHOD FOR PREPARING CORNEAL ENDOTHELIAL CELL |
摘要 |
The present invention relates to a method for preparing corneal endothelial progenitor cells by adherent culturing a cell population isolated from corneal endothelial cell tissue at a low density by using a serum-free medium. The present invention also relates to a method for preparing corneal endothelial cells by differentiation-inducing the corneal endothelial progenitor cells obtained by the aforementioned method. According to the present invention, corneal endothelial progenitor cells can be selectively grown from a corneal tissue-derived cell population, and corneal endothelial cells obtained by inducing the corneal endothelial progenitor cells can be applied to treatment of corneal endothelial diseases. As a result, problems of corneal transplantation such as shortage of donors and occurrence of rejection can be solved. |
申请公布号 |
US2014170751(A1) |
申请公布日期 |
2014.06.19 |
申请号 |
US201214233056 |
申请日期 |
2012.07.13 |
申请人 |
Hayashi Ryuhei;Hara Susumu;Kageyama Tomofumi;Nishida Kohji |
发明人 |
Hayashi Ryuhei;Hara Susumu;Kageyama Tomofumi;Nishida Kohji |
分类号 |
C12N5/079 |
主分类号 |
C12N5/079 |
代理机构 |
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代理人 |
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主权项 |
1. A method for preparing corneal endothelial progenitor cells in which a cell population isolated from corneal endothelial cell tissue is adherent cultured at a low density of 5000 cells/cm2 or less by using a serum-free medium, thereby obtaining the corneal endothelial progenitor cells.
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地址 |
Osaka JP |