DNA POLYMERASES WITH IMPROVED ACTIVITY

摘要

Disclosed are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.

主权项

1. A DNA polymerase having increased reverse transcriptase efficiency compared with a control DNA polymerase, wherein the amino acid of the DNA polymerase corresponding to position 763 of SEQ ID NO:1 is any amino acid other than M or I, and wherein the control DNA polymerase has the same amino acid sequence as the DNA polymerase except that the amino acid of the control DNA polymerase corresponding to position 763 of SEQ ID NO:1 is M or I.