COMPOSITIONS AND METHODS FOR CREATING ALTERED AND IMPROVED CELLS AND ORGANISMS

摘要

The present invention provides compositions comprising randomized in-frame fusion polynucleotides and methods for introducing them into a host organism to identify desirable phenotypic changes that disrupt or alter existing genetic or biochemical mechanisms or pathways, thus creating novel characteristics of the transformed organism. Methods for using the compositions for increasing diversity within populations of organisms are also presented.

主权项

1. A collection of randomly fused polynucleotides, each randomly fused polynucleotide comprising a unique coding region, wherein the unique coding region comprises two complete nonhomologous polynucleotide coding regions of 90-2,502 nucleotides in length operably joined in-frame via a linker sequence.