METHOD OF PRODUCING BIOLOGICALLY ACTIVE VITAMIN K DEPENDENT PROTEINS BY RECOMBINANT METHODS
摘要
The invention relates to commercially viable methods for producing biologically active vitamin K dependent proteins, particularly Factor IX. Factor IX is produced at a level of at least about 15mg/L and is at least 25% biologically active. The method relies upon co-expression of one or more of paired basic amino acid converting encyme (PACE), vitamin K dependent epoxide reductase (VKOR) and vitamin K dependent ³-glutamyl carboxylase (VKGC) at a preferred ratio so that the vitamin K dependent protein is efficiently produced and processed by a recombinant cell.
申请公布号
EP1969127(B1)
申请公布日期
2014.06.18
申请号
EP20060848864
申请日期
2006.12.21
申请人
CNJ HOLDINGS, INC;UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL
发明人
DROHAN, WILLIAM, N.;GRIFFITH, MICHAEL, J.;TAYLOR, JOHN, R.;STAFFORD, DARREL, W.