发明名称 |
Conjugation process of bacterial polysaccharides to carrier proteins |
摘要 |
Process for conjugation of bacterial saccharides including Streptococcus pneumoniae and Haemophilus influenzae saccharides by reductive amination are provided herein. |
申请公布号 |
US8753645(B2) |
申请公布日期 |
2014.06.17 |
申请号 |
US201113581824 |
申请日期 |
2011.03.07 |
申请人 |
GlaxoSmithKline Biologicals S.A. |
发明人 |
Biemans Ralph Leon;Duvivier Pierre;Gavard Ollivier Francis Nicolas |
分类号 |
A61K39/385;C07K1/08;C07K1/113 |
主分类号 |
A61K39/385 |
代理机构 |
|
代理人 |
Bradney Alice P. |
主权项 |
1. A process for conjugating a bacterial saccharide and reducing the sizing effect on bacterial saccharide comprising the steps of
a) reacting the bacterial saccharide with 0.001-0.7 molar equivalents of periodate to form an activated bacterial saccharide, b) mixing the activated bacterial saccharide with a carrier protein; c) reacting the activated bacterial saccharide and the carrier protein with a reducing agent to form a conjugate; wherein step a) occurs in a buffer which does not contain an amine group, and the buffer has a concentration between 1-100 mM and wherein the bacterial saccharide is S.pneumoniae capsular saccharide 23F.
|
地址 |
Rixensart BE |