Method for detoxifying a biological tissue

摘要

Biological tissues may be prepared for use in biological prostheses. The biological tissue may be fixated with glutaraldehyde and may be subjected to successive treatment of the tissue with a solution containing taurine to neutralize excess aldehyde groups that remain free after fixation.

主权项

1. A method of treating a biological tissue for use in a biological prosthesis, the method comprising the steps:
fixation of the biological tissue via a treatment with a solution containing about 0.5% v/v glutaraldehyde in phosphate buffer at room temperature and a pH of 7.4; detoxification of the fixed biological tissue via a treatment with a solution containing about 0.70% w/v taurine in phosphate buffer at a temperature between 40° C. and 50° C. and a pH about 7; and washing of the detoxified fixed biological tissue in phosphate buffer at room temperature and a pH of 7; wherein the detoxification of the fixed biological tissue at a temperature greater than room temperature reduces the number of free aldehyde groups present on the fixed biological tissue when compared to detoxification of fixed biological tissue at room temperature.