Method of assaying 1,5 anhydroglucitol by using whole blood and measurement kit

摘要

In quickly assaying a blood component interfered by glucose and/or its derivative on the bedside or in a clinic or in assaying the same by a patient in his/her own home, there has been required an assay method wherein the whole blood can be used as a sample as such without resorting to a centrifuge or the like. A method of assaying a blood component to be used for assaying a blood component interfered by glucose and/or its derivative, characterized by comprising bringing the whole blood into contact with a substance capable of converting glucose and/or its derivative into another substance not interfering the assay and, simultaneously or subsequently, separating blood cells; a device to be used in the assay method; and a kit containing this device.

主权项

1. A method for measuring 1,5-anhydroglucitol that is subject to interference caused by glucose and/or a derivative thereof, which comprises the steps of:
bringing a whole blood sample collected from a subject directly into contact with a substance converting glucose and/or a derivative thereof to a substance that does not interfere with the measurement of 1,5-anhydroglucitol, wherein the substance converting glucose and/or a derivative thereof to a substance that does not interfere with the measurement of 1,5-anhydroglucitol is a substance for enzymatic oxidation or enzymatic phosphorylation of glucose; thereafter isolating blood cells from the whole blood sample by using a filtration material for blood cell separation; and thereafter measuring 1,5-anhydroglucitol in the sample.