Method for microbes depletion in human blood or full serum using antimicrobial photodynamic laser therapy

摘要

Treatment methods/devices are provided for attenuating/inactivating the pathogenic microbes found in biological fluids e.g. blood/blood products including human single-donor-fresh-frozen-plasma, platelet concentrate, red blood cells, blood clotting factors. An Antimicrobial Photodynamic Therapy method is used to eliminate multiple (resistant) bacteria, viral agents, fungi, parasites and other undetected or non-easily detected pathogenic microbes or particles in blood and blood products without affecting their biological properties. Resistant bacteria are difficult to be eliminated. This is especially true in the case for S. aureus and related strains, Staphylococcus epidermidis or Propionibacterium acnes, Borrelia species and other bacteria found on skin. Further embodiments eliminate undetected or non-easily detected viral agents contaminating blood/blood products responsible for spreading hepatitis, Acquired ImmunoDeficiency Syndrome and other blood borne viral diseases. Human Immunodeficiency, hepatitis B and hepatitis C viruses have emerged as major blood borne infections. Numerous parasites transmitted through bloods and derived products are also eliminated by these processes/devices.

主权项

1. An antimicrobial photodynamic therapy method for the depletion, elimination, or inactivation of microbe-infused blood and blood products without adversely affecting the essential elements of their biological activity comprises the steps of:
a. collecting a blood; b. separating whole blood into blood components if necessary; c. mixing a photosensitizer with blood or blood components comprising a treatment fluid in a sterile light-transparent container bag; d. placing said container bag into a treatment device bag holder; e. setting said holder into sliding motion, rotating motion or a combination thereof; f. illuminating said treatment fluid at a wavelength appropriate for the selected photosensitizer to form active microbe-free treatment fluid; g. depleting excess and/or un-activated photosensitizer and/or residual fragments by passing the active microbe-free treatment fluid through a photosensitizer-absorber unit comprising a plastic housing filled with tiny porous beads and larger sponges; and h. collecting and storing the treatment fluid, after undergoing steps f and g, in a sterile fashion until further use.