| 发明名称 | GFAbs: GFP-based biosensors possessing the binding properties of antibodies | ||
| 摘要 | A family of GFP scaffolds capable of accommodating two proximal, randomized binding loops is disclosed. GFP-based binders binding with nanomolar affinity are developed from a library of these GFP scaffolds. | ||
| 申请公布号 | US8754193(B2) | 申请公布日期 | 2014.06.17 |
| 申请号 | US201213462123 | 申请日期 | 2012.05.02 |
| 申请人 | Wisconsin Alumni Research Foundation | 发明人 | Shusta Eric V.;Pavoor Tej |
| 分类号 | C07K17/00;C40B40/00 | 主分类号 | C07K17/00 |
| 代理机构 | Quarles & Brady, LLP | 代理人 | Quarles & Brady, LLP |
| 主权项 | 1. A mutated fluorescent protein of either green fluorescent protein (GFP) or green fluorescent protein variants, wherein the mutated fluorescent protein comprises GFP mutations D19N, F64L and A87T, wherein the mutated fluorescent protein fluoresces when a heterologous amino acid sequence SEQ ID NO. 26 is inserted at insertion sites between Glu172-Asp173 and a heterologous amino acid sequence SEQ ID NO. 25 is inserted at insertion sites between Asp102-Asp103. | ||
| 地址 | Madison WI US | ||