Bear bile macromolecular extract and preparation method and use thereof

摘要

A bear bile macromolecular extract with anti-HCV virus function is disclosed. A preparation method comprises the following steps of: taking fresh bear bile or dissolving bear bile powder with water, centrifuging it by a molecular sieve filter membrane with molecular weight cut-off of 100,000 or an ultrafiltration membrane, filtering to obtain sediment, dissolving the sediment with water, adding the solution to sephadex column, separating the solution by using water or buffer as elution solvent, and freeze-drying the eluent to obtain the bear bile macromolecular extract. Experiments show that the bear bile macromolecular extract has anti-HCV virus function and can be used for treating hepatitis C.

主权项

1. A bear bile macromolecular extract with anti-hepatitis C virus function, characterized in that the extract is prepared by the following method:
add water to dissolve bear bile powder to obtain aqueous solution of bear bile in concentration of 10˜100 mg/ml, centrifuge the solution by a molecular sieve filter membrane with molecular weight cut-off of 100,000 at the centrifugal force of 4000×g for 10˜60 minutes, or centrifuge fresh bear bile by a molecular sieve filter membrane with molecular weight cut-off of 100,000 at the centrifugal force of 4000×g for 1˜2 hours; filter to obtain a sediment, dissolve the sediment in water to obtain a solution, add the solution to a pretreated cross-linked dextran column having a dry particle size of 40 μm-120 μm and a globular protein fractionation range of 4×103-1.5×105, separate the solution by using water or buffer as elution solvent, collect the eluent, including the void volume eluent, with absorption at 280 nm, freeze-dry the collected eluent to obtain the bear bile macromolecular extract.