Methods of amplification, purification and detection of nucleic acid sequences especially RNA are described. One aspect of the method involves the hybridisation and subsequent ligation of a nucleic acid structure to the nucleic acid sequence desired to be manipulated. The methods require that the nucleic acid structure comprises a double stranded region and a single stranded region. The single stranded region is complementary to the RNA sequence of interest. The double stranded region may also contain additional functionalities which are then used subsequently in the method.
申请公布号
CA2592425(C)
申请公布日期
2014.06.17
申请号
CA20052592425
申请日期
2005.12.22
申请人
GE HEALTHCARE BIO-SCIENCES CORP.
发明人
NELSON, JOHN R.;DUTHIE, R. SCOTT;DHULIPALA, ROHINI;GROSSMANN, GREGORY A.;SEKHER, ANURADHA