Microorganisms and methods for the biosynthesis of adipate, hexamethylenediamine and 6-aminocaproic acid

摘要

The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

主权项

1. Method for preparing 6-aminocaproic acid, wherein the 6-aminocaproic acid is prepared from 2-oxoheptane-1,7-dioate (2-OHD), using at least one biocatalyst, wherein the method further comprises converting adipate semialdehyde to the 6-aminocaproic acid or converting the 2-OHD to 2-aminoheptane-1,7-dioate (2-AHD), using at least one biocatalyst, wherein the biocatalyst comprises an enzyme capable of catalysing a transamination and/or a reductive amination or a decarboxylation.