发明名称 Methods for selecting oocytes and competent embryos with high potential for pregnancy outcome
摘要 The present invention relates to a method for selecting a competent oocyte or a competent embryo.
申请公布号 US8754014(B2) 申请公布日期 2014.06.17
申请号 US201013264589 申请日期 2010.04.09
申请人 Institut National de la Sante et de la Recherche Medical (INSERM) 发明人 Hamamah Samir;De Vos John;Assou Said
分类号 C12Q1/68;C40B30/04 主分类号 C12Q1/68
代理机构 Whitham Curtis Christofferson & Cook, PC 代理人 Whitham Curtis Christofferson & Cook, PC
主权项 1. A method for selecting an oocyte, that when fertilized produces a viable embryo with a high implantation rate leading to pregnancy, comprising the steps of measuring the expression level of 45 genes, using a technique selected from the group consisting of hybridization, amplification, electrophoresis, immunoassay, and immunohistochemical assay, in a cumulus cell surrounding said oocyte, wherein said genes are WNT6, LRCH4, PAX8, CABP4, PDE5A, BCL2L11, PCK1, TCF20, SLAMF6, EPOR, CACNG6, NLRP1, PECAM1, NOS1, ATF3, KRTAP8, GRIK5, SLC24A3, SLC5A12, SLCA10A2, SLCO1A2, SLC25A5, MG29, NLGN2, PRKACA, FOSB, SIAT6, LOXL2, PRF1, ADPRH, APBB3, EGR3, CNR2, IFITM1, PLA2G5, CAMTA1, SOX4, NFIB, NFIC, RBMS1, GOS2, FAT3, SLC40A1, GPC6 and IGF1R, and comparing said expression levels of said genes in said cumulus cell to reference values obtained from a control, wherein the control is a sample comprising cumulus cells associated with an oocyte, wherein detecting differences in expression levels of said genes in said cumulus cell and said control is predictive of implantation rate, and selecting an oocyte for fertilization and implantation based on a positive prediction of said high implantation rate.
地址 Paris FR