Method and apparatus for assay for multiple analytes

摘要

A method and apparatus for assay of multiple analytes. The method uses a sensing element comprising a substrate upon which is arranged a multiplicity of recognition elements, such that each element is laid out in a predetermined pattern. Each pattern is unique in that it can give rise to a characteristic diffraction pattern in the assay. The patterns may or may not be interpenetrating on the substrate surface. The method of detecting multiple analytes includes contacting the medium of analytes with the patterned substrate, illuminating the substrate by a light source, and detecting any resultant diffraction image. The pattern of diffraction and the intensity of the diffracted signal provides information about the existence of specific analytes and their quantification.

主权项

1. A sensing element for use in a light diffraction assay for detecting the presence or absence of at least two analytes, comprising:
a substrate including opposed surfaces including analyte-specific receptor patterns formed on both of said opposed surfaces, said patterns formed on each opposed surface including a first pre-selected pattern of first analyte-specific receptors and at least a second pre-selected pattern including second analyte-specific receptors, wherein each of said pre-selected patterns on said surface is distinct from the other pre-selected pattern of analyte-specific receptors formed on the surface and, when bound to an analyte, gives rise to a pre-selected diffraction pattern distinct from diffraction patterns formed from all other unbound and bound pre-selected patterns on the surface.