| 摘要 |
Disclosed is a method of amplifying a polynucleotide, comprising:
(a) mixing primers for amplifying the polynucleotide, a polymerase, nucleotide substrates and a template polynucleotide, and
(b) amplifying the polynucleotide by a polymerase reaction,
wherein the polymerase has an amino acid sequence consisting of SEQ ID NO:1 or an amino acid sequence homologous to SEQ ID NO:1, and wherein the amino acid residue corresponding to position 651 of the amino acid sequence has been replaced with glutamic acid. |
