| 摘要 |
There is disclosed an isolated nucleic acid molecule encoding a human neurotrophic growth factor designated enovin and having the amino acid sequence illustrated in FIG. 1, 21, 23 or 24 or encoding a functional equivalent, derivative or bioprecursor of said growth factor. The growth factor preferably comprises the amino acid sequence from position 27 to 139 of the sequence illustrated in FIG. 1, or a functional equivalent, derivative or bioprecursor thereof. The nucleic acid molecule encoding enovin can be used to transform a host cell, tissue or organism by including it in an appropriate vector. The host cell, tissue or organism and the vector also form part of the invention. |
| 主权项 |
1. A method for treating neuropathic pain involving a GFRα-3 receptor in a subject comprising administering to the subject an effective amount of a human neurotrophic growth factor polypeptide comprising:
(a) seven conserved cysteine residues at positions 16, 43, 47, 80, 81, 109, and 111 when numbered in accordance with SEQ ID NO: 3; (b) amino acid residues as follows: C at position 16, L at position 18, V at position 25, L at position 28, G at position 29, L at position 30, G at position 31, E at position 36, F at position 40, R at position 41, F at position 42, C at position 43, G at position 45, C at position 47, C at position 80, C at position 81, Rat position 82, P at position 83, F at position 91, D at position 93, S at position 105, A at position 106, C at position 109 and C at position 111, each when numbered in accordance with SEQ ID NO: 3; and (c) an LGLG (SEQ ID NO: 52) repeat, an FRFC (SEQ ID NO: 53) motif, a QPCCRP (SEQ ID NO: 54) motif, and a SATACGC (SEQ ID NO: 55) motif; wherein said neurotrophic growth factor polypeptide is at least 90% homologous to the amino acid sequence of SEQ ID NO: 3; and wherein said neurotrophic growth factor polypeptide exerts its effect by binding to GFRα-3 receptor, thereby treating neurotrophic pain involving the GFRα-3 receptor.
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