METHOD FOR DETECTING TARGET PARTICLE

摘要

This method for detecting a target particle comprises (a) preparing a solution containing a target particle, a luminescent probe that binds to the target particle and a particle for separation and recovery, or containing the target particle bound to the luminescent probe, the luminescent probe and the particle for separation and recovery, and forming a complex composed of the target particle, the luminescent probe and the particle for separation and recovery in the solution, (b) recovering the particle for separation and recovery from the solution by solid-liquid separation treatment after the (a) and preparing a sample solution containing the particle for separation and recovery, and (c) calculating the number of the complex present in the sample solution according to a scanning molecule counting method, wherein the particles for separation and recovery bind to a complex composed of the target particles and the luminescent probe.

主权项

1. A method for detecting a target particle, comprising:
(a) preparing a solution containing a target particle, a luminescent probe that binds to the target particle, and a particle for separation and recovery that is capable to be bound to a complex composed of the target particle and the luminescent probe, or containing the target particle bound to the luminescent probe, the luminescent probe and the particle for separation and recovery, and forming a complex comprising the target particle, the luminescent probe and the particle for separation and recovery in the solution, (b) recovering the particle for separation and recovery from the solution by solid-liquid separation treatment after (a), and preparing a sample solution containing the particles for separation and recovery, and (c) calculating the number of the complex present in the sample solution prepared in (b), thereby detecting the target particle;the particle for separation and recovery binds to a complex composed of the target particle and the luminescent probe in (a), andthe calculating of the number of the complex in step (c) being carried out by:
detecting light released from the complex in the photodetection region, while moving the location of the photodetection region of the optical system in the sample solution using the optical system of a confocal microscope or multi-photon microscope, individually detecting the complex from the detected light by individually detecting optical signals from individual complexes dispersed and randomly moving in the solution, and counting the number of target particles detected during movement of the location of the photodetection region by counting the number of individually detected complexes.