RECOGNITION-RELEASE NANOPOROUS SUBSTRATE COMPRISING ACTIVE AGENTS, METHODS OF THEIR PREPARATION AND USES

摘要

The present invention relates to controlled release of a substance from a porous substrate (such as mesoporous silica nanoparticles). The particles comprise a guest molecule (e.g. a pharmaceutical agent or a contrast agent) entrapped within the pores of said substrate. The pores are capped by at least one nucleic acid sequence and the agent is released by a triggered reaction of said capping sequence with at least one analyte (biomarker) thereby allowing said capping to be cleaved from said pore. The capping agent can be a DNAzyme that cleaves its substrate and releases the guest molecules upon binding with an analyte. Alternatively the capping agent can be a hairpin oligonucleotide that is digested by a nuclease after hybridization with a complementary nucleic acid analyte. The invention further relates to methods of manufacturing said substrate, uses thereof for the controlled administration of active agents and diagnostic of conditions in a patient.