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1. A cell culture device comprising: a culture vessel provided with a cover, wherein there is situated at least one first zone and at least one second zone, wherein said first zone is a transfer zone for the culture medium essentially containing no cells and said second zone is a cell culture zone, means of circulating the culture medium, allowing circulation of the culture medium through the culture zone, said culture zone comprising a bottom wall and a top wall, wherein each wall is provided with orifices allowing a transfer of culture medium essentially free from cells, characterised in that: it also comprises at least one third and at least one fourth zone, wherein both are culture medium transfer zones essentially free from cells, wherein said third zone is in medium communication with the first and second zone and wherein said fourth zone is in medium communication with the second zone and with the first zone via the culture medium circulation means, and in that the culture medium circulation means allow a circulation of the culture medium from bottom to top in said second culture zone.
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