发明名称 |
SYNTHETIC HEPATITIS C GENOME AND METHODS OF MAKING AND USE |
摘要 |
Synthetic representative HCV subtypes, including a 1a and 1b genome, dubbed Bole1a and Bole1b, are provided using an inventive method of Bayesian phylogenetic tree analysis, ancestral sequence reconstruction and covariance analysis. Bole1a branches centrally among 390 full-genome sequences used in its design, a carefully curated 143 sequence full-genome dataset, and separate genomic regions including an independent set of 214 E1E2 sequences from a Baltimore cohort. Bole1a is phylogenetically representative of widely circulating strains. Full genome non-synonymous diversity comparison and 9-mer peptide coverage analysis showed that Bole1a is able to provide more coverage (94% and 78% respectively) than any other sequence in the dataset including H77, a traditional reference sequence. Bole1a also provides unsurpassed epitope coverage when compared to all known T cell epitopes. |
申请公布号 |
US2014162340(A1) |
申请公布日期 |
2014.06.12 |
申请号 |
US201214114808 |
申请日期 |
2012.05.02 |
申请人 |
Ray Stuart Campbell;Munshaw Supriya;Liu Lin |
发明人 |
Ray Stuart Campbell;Munshaw Supriya;Liu Lin |
分类号 |
C07K14/005;C12N9/02 |
主分类号 |
C07K14/005 |
代理机构 |
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代理人 |
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主权项 |
1. A nucleic acid molecule encoding the genome of a synthetic hepatitis C virus subtype 1a (Bole1a) comprising the nucleotide sequence of SEQ ID NO: 1, or portions or fragments thereof, or the complement thereof.
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地址 |
Lutherville MD US |