METHOD FOR CONSTRUCTING NUCLEIC ACID SEQUENCING LIBRARY AND APPLICATIONS THEREOF

摘要

Provided are a method for constructing a nucleic acid sequencing library and applications thereof. The method for constructing a nucleic acid sequencing library comprises: fragmentizing a nucleic acid sample to obtain a DNA fragment; performing end repair on the DNA fragment, to obtain the DNA fragment after end repair; adding a basic group A at an end of the DNA fragment after end repair, to obtain the DNA fragment with a cohesive end A; connecting two ends of the DNA fragment with the cohesive end A to a first adaptor and a second adaptor respectively, to obtain a connection product with adaptors; amplifying the connection product with the adaptors by using a 5' primer and a 3' primer, to obtain amplified products; and separating the amplified products, and constructing a nucleic acid sequencing library with the amplified products, wherein at least one of the first adaptor, the second adaptor, the 5' primer, and the 3' primer comprise a nucleic acid tag, so that the amplified product contains at least one nucleic acid tag.