| 摘要 |
A method for producing a cellulase, comprising steps (1) to (3) mentioned below, wherein the resultant cellulase can be used effectively in washing agents and for the hydrolysis of starches, the hydrolysis of celluloses and so on: (1) filtrating an aqueous solution of a cellulase originated from a filamentous fungus through an ultrafiltration membrane having a cut-off molecular weight of 100,000 to 200,000 to produce a filtrate and a concentrated enzyme solution that is a non-filtrate; (2) filtering the filtrate produced in step (1) through a second ultrafiltration membrane having a cut-off molecular weight of 5,000 to 50,000 to produce a second concentrated enzyme solution that is a non-filtrate; and (3) mixing the concentrated enzyme solutions produced in steps (1) and (2) together to produce a cellulase originated from the filamentous fungus. |
