发明名称 |
NOVEL MODIFIED PROTEIN COMPRISING TANDEM-TYPE MULTIMER OF MUTANT EXTRACELLULAR DOMAIN OF PROTEIN G |
摘要 |
<p>The purpose of the present invention is: to provide an excellent protein which is further reduced in the binding property to an Fc region of an immunoglobulin and/or the binding property to an Fab region of the immunoglobulin in a weakly acidic region compared with that of a protein containing an extracellular domain of wild-type protein G, and which still keeps a high antibody-binding activity in a neutral region; and to capture and collect an antibody readily using the protein without denaturing the antibody. The present invention relates to: a protein that is reduced in the binding property to an Fc region of an immunoglobulin and/or the binding property to an Fab region of the immunoglobulin in a weakly acidic region compared with that of a multimer comprising an extracellular domain of wild type one, which is a domain having a binding activity to a protein comprising an Fc region of immunoglobulin G, while keeping a high antibody-binding activity in a neutral region, and also has a binding activity to a protein comprising the Fc region of immunoglobulin G, wherein the protein comprises a tandem-type multitimer of a mutant of the extracellular domain; and others.</p> |
申请公布号 |
EP2740792(A1) |
申请公布日期 |
2014.06.11 |
申请号 |
EP20120819336 |
申请日期 |
2012.08.03 |
申请人 |
NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCEAND TECHNOLOGY;DAICEL CORPORATION |
发明人 |
HONDA, SHINYA;MATSUMARU, HIROYUKI;WATANABE, HIDEKI;YOSHIDA, CHUYA |
分类号 |
C12N15/09;C07K14/315;C07K17/00;C07K19/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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