SEPARATING AGENT FOR PURIFICATION OF PROTEIN, AND PROTEIN PURIFICATION METHOD

摘要

A novel separating agent for protein purification which not only can adsorb proteins in a sufficient amount for protein purification from a low concentration buffer but also can desorb the adsorbed protein easily just by altering the pH of the buffer and a simple and economical method for its production and a method for protein purification using it. One or two ligands selected from the group consisting of a ligand represented by the following formula (1):
(wherein m is an integer of from 2 to 6) and a ligand represented by the following formula (2):
(wherein each of R 1 and R 2 is independently a hydrogen atom or a C 1-4 alkyl group, and n is an integer of from 1 to 6) are immobilized on a support via a urethane bond without intervention of a spacer arm.