TRANSFORMED SACCHAROMYCES CEREVISIAE FOR IMPROVING THE CONTENT OF FATTY ACIDS AND PREPARATION METHOD THEREOF

摘要

The present invention relates to transformed yeast for improving the content of fatty acids and a preparation method thereof and provides the transformed yeast for producing fatty acids which is transformed with a recombination vector comprising; (i) a nucleotide sequence which codes an oxoacyl carrier protein synthetase II (fabF) derived from Saccharomyces cerevisiae; (ii) a nucleotide sequence which codes oxoacyl carrier protein reductase (fabG) derived from Streptococcus pyogenes; (iii) a nucleotide sequence which codes a hydroxyl myristoyl carrier protein dehydratase (fabZ) derived from Streptococci pyogenes; (iv) a nucleotide sequence which codes ynoyl carrier protein reductase II (fabI) derived from Streptococcus pyogenes; or (v) a nucleotide sequence which codes oleyl carrier protein hydrolase (EC 3.1.2.14) derived from Streptococcus pyogenes. The present invention increases the content of the fatty acids itself by over-expressing an extension step of a fatty acid biosynthesis path by Saccharomyces cerevisiae and mass-produces fatty acid biosynthesis through a metabolism flow change due to the over-expression of the acid biosynthesis path from glucose and the insertion of other species.