发明名称 |
PCSK9 immunoassay |
摘要 |
Methods of using PCSK9 antagonists More specifically, methods for measuring circulating PCSK9 levels in a biological sample by means of an immunoassay The immunoassay used can be a solid phase immunoassay, such as a dissociation-enhanced lanthanide fluorescence immunoassay utilizing an E07 capture antibody or coating and a G08 or H23 detecting antibody. |
申请公布号 |
US8748115(B2) |
申请公布日期 |
2014.06.10 |
申请号 |
US200913139045 |
申请日期 |
2009.12.02 |
申请人 |
Merck Sharp & Dohme Corp. |
发明人 |
Ni Yan;Sitlani Ayesha;Pandit Shilpa;Lewis Dale;Shen Xun;Lobo Sharon;McCabe Timothy;Condra Jon;Cubbon Rose |
分类号 |
G01N33/573;G01N33/543;C07K16/40 |
主分类号 |
G01N33/573 |
代理机构 |
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代理人 |
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主权项 |
1. A method of measuring circulating Proprotein Convertase Subtilisin-Kexin 9 (PCSK9) levels in a biological sample obtained from a subject, comprising the steps of performing a solid phase immunoassay on the biological sample to determine a level of PCSK9 therein and comparing the level of PCSK9 in the biological sample against a standard having a known concentration of PCSK9, wherein the immunoassay is performed using a coating or capture antibody that is E07 and a detecting antibody that is H23; and wherein:
a) the E07 antibody comprises a light chain consisting of the amino acid sequence of SEQ ID NO: 3 or 7, and a heavy chain consisting of the amino acid sequence of SEQ ID NO: 4 or 8; and b) the H23 antibody comprises a light chain consisting of the amino acid sequence of SEQ ID NO: 11 or 15, and a heavy chain consisting of the amino acid sequence of SEQ ID NO: 12 or 16.
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地址 |
Rahway NJ US |