Methods, compositions, and devices utilizing MicroRNA to determine physiological conditions

摘要

Methods, compositions, and devices are disclosed which use microRNA to detect, predict, treat, and monitor physiological conditions such as disease or injury. microRNA are isolated and their differential expression is measured to provide diagnostic information. This information may then be utilized for evaluation and/or treatment purposes.

主权项

1. A method of detecting or predicting a liver condition, comprising:
generating a microRNA profile from a biological sample, wherein the microRNA profile comprises the amounts of at least two specific microRNA sequences, by:
receiving the biological sample, the sample being a tissue, serum, or plasma; andisolating microRNA from the biological sample by extracting the microRNA with an organic solvent and obtaining an aqueous phase containing the microRNA, then purifying the aqueous phase through a silica membrane to isolate the microRNA;using hybridization to identify the microRNA sequences; andmeasuring the amount of at least two specific microRNA sequences; and comparing the amounts of the at least two specific microRNA sequences to a reference to provide information for detecting or predicting the liver condition; wherein the at least two specific microRNA sequences are hsa-miR-122 and hsa-miR-486-3p.