| 摘要 |
FIELD: medicine, pharmaceutics.SUBSTANCE: invention refers to preparing recombinant proteins and concerns methods for preparing them. There are presented methods for preparing antibodies, involving: culturing mammalian cells containing nucleic acid coding an antibody or its fragment in a cell culture containing a production medium of the cell cultures; feeding the mammalian cells by adding a hydrolysate-rich solution and a rich solution of a basal medium to the cell culture, wherein the hydrolysate-rich solution contains a herbal hydrolysate and a yeast hydrolysate 75-300 g/ml. The presented methods can be used to prepare particularly such antibodies, as anti-TNF?-antibody or anti-IL-12-antibody.EFFECT: characterised solution enables preparing high antibody count and may be used in pharmaceutical industry.28 cl, 46 tbl, 4 ex, 1 dwg |
