摘要 |
FIELD: medicine.SUBSTANCE: invention refers to methods for determining the efficacy of an ion channel ligand. The ex vivo methods for determining the efficacy of the ion channel ligand in vivo depending on plasma, involves the stages as follows: a) contacting a cell expressing the ion channel with i) animal's plasma and ii) the ion channel ligand and b) determining the effect of the ion channel ligand on the cell or a) contacting the cell expressing the ion channel with i) animal's plasma and ii) a compound that is defined as the ion channel ligand, and b) determining the effect of the compound on the cell, or a) contacting the cell expressing the ion channel with animal's plasma wherein the ion channel ligand has been administered, and b) determining the effect of the ion channel ligand on the cell. The method according to the invention may be used for screening of a therapeutic preparation for preventing and/or treating a disease involving the ion channel dysfunction, especially for preventing and/or treating a cardiovascular disease or cancer.EFFECT: invention provides higher effectiveness of the method and lower costs of screening of therapeutic preparations.14 cl, 4 tbl, 2 ex, 3 dwg |