Apparatus for desorption and ionization of analytes

摘要

This invention relates generally to methods and apparatus for desorption and ionization of analytes for the purpose of subsequent scientific analysis by such methods, for example, as mass spectrometry or biosensors. More specifically, this invention relates to the field of mass spectrometry, especially to the type of matrix-assisted laser desorption/ionization, time-of-flight mass spectrometry used to analyze macromolecules, such as proteins or biomolecules. Most specifically, this invention relates to the sample probe geometry, sample probe composition, and sample probe surface chemistries that enable the selective capture and desorption of analytes, including intact macromolecules, directly from the probe surface into the gas (vapor) phase without added chemical matrix.

主权项

1. A probe that is removably insertable into a mass spectrometer, the probe having a surface for presenting an analyte to an energy source that emits energy capable of desorbing the analyte from the probe for analyte detection, wherein the probe comprises an immobilized metal ion on the probe surface, wherein the metal ion is immobilized on the probe surface by a chelating agent and the metal ion is bound to the analyte, and further comprising a matrix applied to the probe surface.