Non-tumorigenic MDCK cell line for propagating viruses

摘要

The present invention provides novel MDCK-derived adherent non-tumorigenic cell lines that can be grown in the presence or absence of serum. The cell lines of the present invention are useful for the production of vaccine material (e.g., viruses). More specifically, the cell lines of the present invention are useful for the production of influenza viruses in general and ca/ts influenza viruses in particular. The invention further provides methods and media formulations for the adaptation and cultivation of MDCK cells such that they remain non-tumorigenic. Additionally, the present invention provides methods for the production of vaccine material (e.g., influenza virus) in the novel cell lines of the invention.

主权项

1. A process for the production of influenza viruses comprising:
a) infecting adherent and non-tumorigenic Madin Darby Canine Kidney (MDCK) cells with influenza viruses, which cells are adapted to grow and cultivated in serum-free medium comprising Taub's medium, lipids and wheat hydrolysate, which Taub's medium comprises a 50:50 mixture of DMEM and Ham's F12, 4.5 grams per liter glucose, 4 millimolar L-glutamine, 5 micrograms per milliliter insulin, 5 micrograms per milliliter transferrin, 5 picomolar triiodothyronine, 50 nanomolar hydrocortisone, 25 nanograms per milliliter prostaglandin E1 and 10 nanomolar sodium selenite; b) incubating the cells; and c) isolating the influenza viruses from the cells.