Method of detecting fungi belong to genus <i>Geosmithia </i>

摘要

A method of detecting a fungus belonging to genus Geosmithia, including identifying a fungus belonging to genus Geosmithia using a nucleic acid represented by the nucleotide sequence defined in the following (a) or (b):
(a) a partial nucleotide sequence of β-tubulin gene shown in any one of SEQ ID NOS: 1 to 3, or a complementary sequence thereof;(b) a nucleotide sequence including deletion, substitution, insertion or addition of one or several nucleotide(s) in the nucleotide sequence shown in any one of SEQ ID NOS: 1 to 3, or a complementary sequence thereof.

主权项

1. A method of detecting whether a fungus belonging to genus Geosmithia is in a test sample, comprising
(a) hybridizing, under stringent conditions, both (i) an oligonucleotide consisting of SEQ ID NO: 4, or an oligonucleotide consisting of a nucleotide sequence that has 90% or more identity with the nucleotide sequence of SEQ ID NO: 4 and (ii) an oligonucleotide consisting of SEQ ID NO: 5, or an oligonucleotide consisting of a nucleotide sequence that has 90% or more identity with the nucleotide sequence of SEQ ID NO: 5, to nucleic acid in the test sample or to nucleic acid obtained from the test sample; and (b) determining whether there is hybridization of both (i) and (ii) to the nucleic acid; wherein a fungus belonging to the genus Geosmithia is detected in the test sample when both (i) and (ii) hybridize to the nucleic acid.