Methods for Improvement of Enzymatic Hydrolysis of Lignocellullosic Material

摘要

The present invention relates to a method of enzymatic hydrolysis of a lignocellulosic material, comprising the steps of: a) pretreating the lignocellulosic material to obtain a slurry having a pH of less than 6; b) adding NaOH, Ca(OH)2 and/or CaO to the slurry to increase its pH to at least 8, said addition being carried out at a slurry temperature of at least 60° C.; c) reducing the pH of the slurry to below 7; and optionally cooling the slurry from step b) to a temperature below 60° C.; and d) adding hydrolytic enzymes to the slurry from c) and allowing the slurry to hydrolyze wherein no washing of the slurry is performed prior to step d)

主权项

1. A method of enzymatic hydrolysis of a lignocellulosic material, comprising the steps of:
a) pretreating the lignocellulosic material to obtain a slurry having a pH of less than 6; b) adding NaOH, Ca(OH)2 and/or CaO to the slurry to increase its pH to at least 8, said addition being carried out at a slurry temperature of at least 60° C.; c) reducing the pH of the slurry to below 7 and optionally cooling the slurry from step b) to a temperature below 60° C.; and d) adding hydrolytic enzymes to the slurry from step c) and allowing the slurry to hydrolyze, wherein no washing of the slurry is performed prior to step d).