Adaptive Immunity Profiling and Methods for Generation of Monoclonal Antibodies

摘要

Methods are provided for producing monoclonal antibody candidates using adaptive immunity profiling. In some aspects, the method provides for the use of massively parallel signature sequencing.

主权项

1. A method of generating monoclonal antibodies, comprising the steps of:
a) immunizing a host subject with an antigen; b) creating a cDNA sequence library, the creating comprising the steps of:
i) isolating lymphocytes from the host subject;ii) isolating mRNA from the lymphocytes;iii) reverse transcribing the mRNA to cDNA;iv) amplifying target antibody cDNA sequences; andv) sequencing the target antibody cDNA sequences; and c) analyzing the frequency of target antibody cDNA sequences, wherein target antibody cDNA sequences having a relatively high frequency are identified as candidate antibody sequences.