METHOD OF OBTAINING CIRCULATING CANCER CELL POPULATIONS, ASSOCIATED CELLULAR COMPOSITIONS AND METHODS OF USING SAME

摘要

It is herewith provided methods of obtaining circulating cancer cells (CCCs)-enriched cellular populations based on the removal of CD45-positive cells from an apheresis product. It is also provided methods of obtaining circulating stem cells (CSCs)-enriched cellular population from a CCCs-enriched cellular population based on a selection either using the specific cellular markers or through culture. It is further provided methods of obtaining circulating tumor cells (CTCs)-enriched cellular population from a CCCs-enriched cellular population based on a selection using the specific cellular markers. It is also provided screening assays for the selection of (chemo)therapeutic agent as well as personalized medicine application based on drug sensitivity/resistance or cancer markers.

主权项

1. A method of providing a population of cells enriched in circulating cancer cells, the method comprising:
a) depleting an apheresis nucleated cellular product from CD45-positive leukocytes to provide the population of cells enriched in circulating cancer cells; and b) collecting the population of cells enriched in circulating cancer cells.