| 摘要 |
The present invention is directed to methods for purifying a non-human antibody, or antigen binding portion thereof, exhibiting weak binding strength and low binding capacity for Protein A chromatography media. In one aspect, a kosmotropic salt solution is employed to promote the hydrophobic interaction between the non-human antibody, or antigen binding portion thereof, and the Protein A ligand, thereby enhancing the binding of the non-human antibody, or antigen binding portion thereof, to the Protein A chromatography media. In another aspect, the concentration of the non-human antibody, or antigen binding portion thereof, in a sample comprising the antibody, or antigen binding portion thereof, exposed to a Protein A chromatography media is increased to enhance the binding of the non-human antibody, or antigen binding portion thereof, on the Protein A chromatography media. |
| 主权项 |
1. A method for producing a preparation comprising a non-human antibody, or antigen binding portion thereof, and having a reduced level of at least one impurity, said method comprising:
(a) subjecting a sample comprising the non-human antibody, or antigen binding portion thereof, and at least one impurity to a first kosmotropic salt solution; (b) contacting the sample subjected to the kosmotropic salt solution to a Protein A affinity chromatography (PA) media; and (c) obtaining an elution fraction from the Protein A media; wherein the elution fraction comprises the non-human antibody, or antigen binding portion thereof, and has a reduced level of the at least one impurity.
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