Genetically Enhanced Cyanobacteria Lacking Functional Genes Conferring Biocide Resistance for the Production of Chemical Compounds

摘要

One embodiment of the invention provides a genetically enhanced cyanobacterium producing a first chemical compound comprising:
a genetically enhanced genome with a first gene inactivation in a first essential or conditionally essential gene of the cyanobacterium, anda first extrachromosomal plasmid harboring the first essential or conditionally essential gene and at least one first production gene for production of the first chemical compound,wherein the cyanobacterium lacks a functional gene conferring biocide resistance.;These cyanobacteria can produce a first valuable chemical compound in long-term cultures without the need to employ genes conferring biocide resistance.

主权项

1. A genetically enhanced cyanobacterium producing ethanol, comprising:
a) a genetically enhanced genome with a first gene inactivation in a first essential or conditionally essential gene of the cyanobacterium selected from the group consisting of smtAB, leuB, ziaRA, corRT, narB and pyrF; and b) a first extrachromosomal plasmid harboring the first essential or conditionally essential gene and at least one first production gene for the production of ethanol, wherein the at least one first production gene for the production of ethanol encodes an enzyme selected from the group consisting of Adh, Pdc, AdhE and combinations thereof;wherein the cyanobacterium lacks a functional gene conferring biocide resistance, further wherein the genome harbors more than one copy of the first essential or conditionally essential gene and wherein all copies of the first essential gene carry at least one gene inactivation, and further wherein said genetically enhanced cyanobacterium produces ethanol.