Single Cell Nucleic Acid Detection and Analysis

摘要

Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided.

主权项

1. A method of determining copy number of a nucleic acid molecule in a sample including a plurality of nucleic acid molecules comprising
attaching a unique barcode sequence to substantially each of the plurality of nucleic acid molecules in the sample to produce a plurality of barcoded nucleic acid molecules, amplifying the plurality of barcoded nucleic acid molecules in the sample to produce amplicons of the plurality of barcoded nucleic acid molecules, sequencing each amplicon to identify an associated nucleic acid sequence and an associated barcode sequence, selecting a first target nucleic acid sequence and determining the number of unique associated barcode sequences for the first target nucleic acid sequence, wherein the number of unique associated barcode sequences is the copy number of the first target nucleic acid sequence.