发明名称 Active chemically-sensitive sensors with correlated double sampling
摘要 Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
申请公布号 US8742469(B2) 申请公布日期 2014.06.03
申请号 US201213599913 申请日期 2012.08.30
申请人 Life Technologies Corporation 发明人 Milgrew Mark
分类号 G01N27/403 主分类号 G01N27/403
代理机构 代理人
主权项 1. An apparatus, comprising: an array of sensors, a sensor in the array including: a chemically-sensitive field effect transistor (chemFET) having a first conduction terminal, a second conduction terminal coupled to a first supply voltage conductor, and a sensor surface;a first transistor having a first conduction terminal coupled to the first conduction terminal of the chemFET, a second conduction terminal coupled to a second supply voltage conductor, and a gate coupled to a bias voltage conductor; anda second transistor having a gate coupled to a first control signal conductor, a first conduction terminal coupled to the first conduction terminal of the chemFET, and a second conduction terminal coupled to an amplifier node; a reset circuit coupled to the amplifier node and having an input coupled to a second control signal conductor, the reset circuit to reset the amplifier node to a reference voltage in response to a signal on the second control signal conductor; an amplifier having an input connected to the amplifier node and having an output coupled to an output conductor, the amplifier includes: a third transistor having a gate coupled to the amplifier node, a first conduction terminal coupled to a third supply voltage conductor, and a second conduction terminal; anda fourth transistor having a gate coupled to a select signal conductor, a first conduction terminal coupled to the second conduction terminal of the third transistor, and a second conduction terminal coupled to the output conductor; and a controller to cause the amplifier to provide a first output while the amplifier node is reset to the reference voltage, and to cause the amplifier to provide a second output while the chemFET is coupled to the amplifier node.
地址 Carlsbad CA US