Methods for classification of somatic embryos comprising hyperspectral line imaging

摘要

The present invention relates to methods, apparatus, and imaging systems for using near-infrared spectroscopy imaging of plant embryos for classifying plant embryos. In one embodiment, a method is provided for classifying a plant embryo of an unknown type based on near infrared spectroscopy imaging.

主权项

1. A method for classifying plant embryos comprising:
(a) developing a classification model by
(i) acquiring reflectance spectral raw data representative of a known embryo type by obtaining a plurality of line images of a plurality of embryos of the known embryo type, wherein the plurality of line images are taken from a plurality of positions along and substantially perpendicular to a desired axis of ones of the plurality of embryos;(ii) performing a data analysis by applying one or more classification algorithms to the spectral raw data, the data analysis resulting in development of a classification model for classifying plant embryos by embryo type; (b) acquiring, by a computer, reflectance spectral raw data of a plant embryo of unknown embryo type by obtaining a plurality of line images of the plant embryo of unknown embryo type, wherein the plurality of line images are taken from a plurality of positions along and substantially perpendicular to a corresponding axis of the plant embryo of unknown embryo type; and (c) applying the developed classification model to the spectral raw data of the plant embryo of unknown embryo type in order to classify the type of plant embryo of unknown embryo type.