| 发明名称 |
Method of detecting ethylated thymidine DNA adducts |
| 摘要 |
A method of analyzing ethylated thymidine DNA adducts is disclosed. The method comprises the steps of: providing leukocyte DNA; adding at least one isotope-labeled internal standard and a plurality of enzymes to the leukocyte DNA, and hydrolyzing the leukocyte DNA into a plurality of nucleosides; using a solid-phase extraction column to extract the plurality of nucleosides; and using a stable isotope dilution nanoflow liquid chromatography-nanospray ionization tandem mass spectrometry to detect and quantify at least one ethylated thymidine DNA adduct in the plurality of extracted nucleosides. |
| 申请公布号 |
US8741562(B2) |
申请公布日期 |
2014.06.03 |
| 申请号 |
US201213664020 |
申请日期 |
2012.10.30 |
| 申请人 |
National Chung Cheng University |
发明人 |
Chen Hauh-Jyun Candy;Wang Yi-Ching;Lin Wen-Peng |
| 分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
| 代理机构 |
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代理人 |
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| 主权项 |
1. A method of analyzing ethylated thymidine DNA adducts, comprising the steps of:
providing a leukocyte DNA; adding at least one isotope-labeled internal standard and a plurality of enzymes into the leukocyte DNA to hydrolyze the leukocyte DNA into a plurality of nucleosides; using a solid phase extraction column to extract the plurality of nucleosides; and using an isotope dilution nanoflow liquid chromatography-nanospray ionization tandem mass spectrometry (nanoflow LC-NSI/MS/MS) to detect and quantify at least one ethylated thymidine DNA adduct in the plurality of extracted nucleosides.
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| 地址 |
Chia-Yi TW |
