| 摘要 |
An analytic method uses a labeled antigen, labeled antibody, tracer, etc. in which a radio isotope, fluorescent dye, luminescent dye, enzyme, etc. is bonded to an antibody or an antigen bonded to a protein as an object of analysis for performing an assay of a trace amount of a substance such as a hormone, tumor marker, infective pathogen marker, infective antibody, etc., for blood, serum, plasma, or body fluid as a sample or specimen. The analysis is enabled at high sensitivity, with less generation of noises and at low background. When an assay is performed based on the antigen-antibody reaction for a sample in an analytic method by fluorometry or luminometry, detection or measurement of non-specific fluorescence or luminescence such as interference light is eliminated or removed effectively irrespective of the property of the sample or the level of the concentration of substances contained in the sample. |
| 主权项 |
1. An automatic analyzer comprising:
a reaction vessel to contain a reaction solution including antigens and antibodies for an antigen-antibody reaction, where luminescent labels are bonded to one of the antigens, the antibodies or another substance in the reaction solution directly or indirectly, the reaction vessel includes a portion which collects carriers formed with antigen-antibody bonding present in the reaction solution from the antigen-antibody reaction, the carriers including the luminescent labels; a measuring mechanism to detect light generated from the carriers collected on the portion of the reaction vessel; and a moving mechanism to move the reaction vessel to a measurement position and the portion of the reaction vessel which has collected the carriers formed with the antigen-antibody bonding are separated from a remainder of the reaction solution so that the measuring mechanism detects the light from the portion of the reaction vessel, wherein the moving mechanism moves the reaction vessel to the measurement position a plurality of times after starting the antigen-antibody reaction so that the measuring mechanism quantitatively measures the antigen-antibody reaction in time series, and wherein the concentration of carriers collected at the portion of the reaction vessel changes over the time series.
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