Cell lines and their use in encapsulated cell biodelivery

摘要

The present invention relates to generation of cell lines expressing recombinant proteins for use in naked and encapsulated cell biodelivery of secreted therapeutic molecules. In one embodiment the cell line is human. In another aspect of the invention the transposon system is used for generating a cell line for secretion of a biologically active polypeptide.

主权项

1. A monoclonal cell line comprising a transposon, said transposon comprising two terminal inverted repeats and a heterologous expression construct coding for a structural gene either
i coding for a secreted biologically active polypeptide or ii coding for a polypeptide or an siRNA contributing to the generation in the cells of a biologically active secreted compound;said gene being located between said two terminal inverted repeats which are substrates for a transposase and said transposon being inserted into the genome of said monoclonal cell line using said transposase, wherein said monoclonal cell line maintains a high and stable expression level of the polypeptide or siRNA of I or ii absent the presence of a selective pressure compared to the same monoclonal cell line generated without a transposase.