Amnion-derived cell compositions, methods of making and uses thereof

摘要

The invention is directed to substantially purified amnion-derived cell populations, compositions comprising the substantially purified amnion-derived cell populations, and to methods of creating such substantially purified amnion-derived cell populations, as well as methods of use. The invention is further directed to antibodies, in particular, monoclonal antibodies, that bind to amnion-derived cells or, alternatively, to one or more amnion-derived cell surface protein markers. The invention is further directed to methods for producing the antibodies, methods for using the antibodies, and kits comprising the antibodies.

主权项

1. A composition comprising conditioned media, wherein the conditioned media is made by a method comprising the steps of
a) obtaining a placenta and isolating an amnion from the placenta, b) enzymatically releasing amnion-derived epithelial cells from the amnion, c) collecting the released amnion-derived epithelial cells, d) culturing the collected amnion-derived epithelial cells of step (c) in basal culture medium that is supplemented with human serum albumin and recombinant human EGF; e) removing the culture medium after about 2-3 days and applying fresh culture medium; and f) collecting the culture medium after culturing the cells for 2-3 days.