Production of oil seed protein isolate

摘要

Oil seed protein isolates, particularly canola protein isolate, are produced at a high purity level of at least about 100 wt % (N×6.25) by a process wherein oil seed protein is extracted from oil seed meal, the resulting aqueous protein solution is concentrated to a protein content of at least about 200 g/L and the concentrated protein solution is added to chilled water having a temperature below about 15° C. to form protein micelles, which are settled to provide a protein micellar mass (PMM). The protein micellar mass is separated from supernatant and may be dried. The supernatant may be processed to recover additional oil seed protein isolate by concentrating the supernatant and then drying the concentrated supernatant, to produce a protein isolate having a protein content of at least about 90 wt %. The concentrated supernatant may be mixed in varying proportions with at least part of the PMM and the mixture dried to produce a protein isolate having a protein content of at least about 90 wt %.

主权项

1. A process of preparing a canola protein isolate, which comprises:
(a) extracting a canola oil seed meal at a temperature of at least about 5° C. to cause solubilization of protein in said oil seed meal and to form an aqueous canola protein solution having a protein content of about 5 to about 25 g/L and a pH of about 5 to about 6.8, (b) separating the aqueous canola protein solution from residual canola oil seed meal, (c) increasing the protein concentration of said aqueous canola protein solution to at least 250 g/L while maintaining the ionic strength substantially constant by using a selective membrane technique to provide a concentrated canola protein solution, (d) diluting said concentrated canola protein solution into chilled water having a temperature below about 15° C. to cause the formation of canola protein micelles, (e) settling the canola protein micelles to form an amorphous, sticky, gelatinous, gluten-like canola protein micellar mass, and (f) separating the canola protein micellar mass from supernatant said canola protein micellar mass having a protein content of at least about 100 wt % as determined by Kjeldahl nitrogen×6.25 on a dry weight basis.