| 摘要 |
Disclosed is a method for detecting Factor D in a diagnostic assay comprising: (a) contacting a cell, tissue or serum sample with an anti-Factor D antibody or an antigen-binding fragment thereof; and (b) detecting binding of the antibody or the antigen-binding fragment thereof to the sample, wherein the binding to the sample indicates the presence of Factor D in the sample; wherein the antibody or the antigen-binding fragment thereof comprises a light chain variable domain and a heavy chain variable domain, wherein the light chain variable domain comprises light chain hyper variable region (HVR)-1 comprising ITSTDIDDDMN (SEQ ID NO: 30), light chain HVR-2 comprising GGNTLRP (SEQ ID NO: 35), and light chain HVR-3 comprising LQSDSLPYT (SEQ ID NO: 38), wherein the heavy chain variable domain comprises heavy chain HVR-1 comprising GYTFTNYGMN (SEQ ID NO: 39), heavy chain HVR-2 comprising WINTYTGETTYADDFKG (SEQ ID NO: 40), and heavy chain HVR-3 comprising EGGVNN (SEQ ID NO: 41), wherein the anti-Factor D antibody or antibody fragment thereof further comprises one or more of the following substitutions (a) to (f): (a) substitution of the amino acid at position 10 of SEQ ID NO: 30 with L or I; (b) substitution of the amino acid at position 11 of SEQ ID NO: 30 with A or Q; (c) substitution of the amino acid at position 3 of SEQ ID NO: 35 with S or A; (d) substitution of the amino acid at position 5 of SEQ ID NO: 41 with A or Q; or (e) substitution of the amino acid at position 6 of SEQ ID NO: 41 with A or Q, and/or (f) the heavy chain variable domain comprises an E at position 1. |
