MOLECULES INHIBITING THE HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-1), METHOD FOR THE PRODUCTION THEREOF AND APPLICATIONS OF SAME

摘要

The invention relates to an aptamer, the structure thereof comprising at least one nucleotide sequence 5'-GGCA(A/G)GGA-3', that can specifically bind to the poly(A) hairpin of the 5'UTR region of the genome of the human immunodeficiency virus type 1 (HIV-1), providing the method for producing aptamers with said sequence by means of a combination of experimental techniques of in vitro selection of nucleic acids with computational techniques of sequence optimisation. The invention also relates to a DNA gene structure for synthesising said aptamers, preferably RNA. The invention further relates to the different uses of the above-mentioned aptamer, including the use thereof as a biosensor molecule for detecting and/or quantifying HIV-1, as an inhibitor of the production of viral particles of HIV-1, and to the application thereof in medicine, the invention also relating to a method for treating a disease caused by HIV-1, and to a pharmaceutical composition comprising said aptamer.